This study aimed to investigate the impact of electronic portfolio on the development of reflective thinking and self -directed learning readiness of the students in the Faculty of Educational Sciences at UNRWA. To achieve this goal, the researchers used a reflective thinking scale, consists of (16) items, and a self directed learning readiness scale, (41) items, and applied them, after investigating their psychometric properties, on a sample of (49) students, from fourth-year faculty students, enrolled in the practice education program, the sample divided into two groups, the experimental group, using the e-portfolio assessment strategy, and the control group, with the traditional assessment strategy, analysis of covariance (ANCOVA) was used. The results of the study showed statistically significant differences between the two groups on reflective thinking and self directed learning readiness, and on the majority of their subscales, in favor of the experimental group. The study ended with some recommendations aimed at activating the e-portfolio strategy, in evaluation, in different areas, and various levels